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tissue dissociation kit  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec tissue dissociation kit
    Tissue Dissociation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2075 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tissue+dissociation+kit/Neural+Tissue+Dissociation+Kit/pmc13418531-398-13-16
    Average 97 stars, based on 2075 article reviews
    tissue dissociation kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation.
    Article Snippet: .. Spinal cord cell isolation Mice were killed, and spinal cords were extracted, cut into small pieces and digested using either a tissue dissociation kit (Miltenyi Biotec, tissue dissociation kit 1 for inflamed neural tissue, 130-110-201) according to the manufacturer’s instructions or 3 ml of digestion medium (DMEM supplemented with 1 mg ml−1 papain (Sigma-Aldrich, P4762), 0.03 mg ml−1 DNase I (Roche, 11284932001) and 0.5 mg ml−1 collagenase/ dispase (Sigma-Aldrich, 10269638001)). .. In experiments where digestion medium was used, mechanical and enzymatic dissociation was performed with a GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) and a spinal cord-specific program (37C_ABDK_01).

    Mouse Assay:

    Article Title: Monocyte infiltration induces CNS arginine catabolism to fuel neuroinflammation.
    Article Snippet: .. Spinal cord cell isolation Mice were killed, and spinal cords were extracted, cut into small pieces and digested using either a tissue dissociation kit (Miltenyi Biotec, tissue dissociation kit 1 for inflamed neural tissue, 130-110-201) according to the manufacturer’s instructions or 3 ml of digestion medium (DMEM supplemented with 1 mg ml−1 papain (Sigma-Aldrich, P4762), 0.03 mg ml−1 DNase I (Roche, 11284932001) and 0.5 mg ml−1 collagenase/ dispase (Sigma-Aldrich, 10269638001)). .. In experiments where digestion medium was used, mechanical and enzymatic dissociation was performed with a GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) and a spinal cord-specific program (37C_ABDK_01).

    Article Title: Interferon‐γ–Responsive Microglia‐Derived Extracellular Vesicles Inhibited Neurogenesis After Stroke via MicroRNA‐199a‐5p/ <i>SIRT1</i> Axis
    Article Snippet: Images were captured by confocal (Leica). .. For quantitative analysis, images were acquired from 10 randomly selected fields at 20× magnification for each cell coverslip, and 3 to 4 representative sections were obtained from each mouse. scRNA- Seq of Mice Brain Fresh mice brain tissues were dissociated into singlecell suspensions using a tissue dissociation kit (Miltenyi, Cologne, Germany), followed by live cell sorting (viability >85%). ..

    FACS:

    Article Title: Interferon‐γ–Responsive Microglia‐Derived Extracellular Vesicles Inhibited Neurogenesis After Stroke via MicroRNA‐199a‐5p/ <i>SIRT1</i> Axis
    Article Snippet: Images were captured by confocal (Leica). .. For quantitative analysis, images were acquired from 10 randomly selected fields at 20× magnification for each cell coverslip, and 3 to 4 representative sections were obtained from each mouse. scRNA- Seq of Mice Brain Fresh mice brain tissues were dissociated into singlecell suspensions using a tissue dissociation kit (Miltenyi, Cologne, Germany), followed by live cell sorting (viability >85%). ..

    Single Cell:

    Article Title: IL-33 is Required for Behavioral Deficits in a Murine Model of Systemic Lupus Erythematosus Through Regulation of Microglia Glycolysis.
    Article Snippet: .. Single-cell suspensions were prepared from the isolated brain tissue using a papain-based neural tissue dissociation kit (Miltenyi Biotec), according to the manufacturer's instructions. .. Single-cell suspensions were prepared from the isolated brain tissue using a papain-based neural tissue dissociation kit (Miltenyi Biotec), according to the manufacturer's instructions.

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Cortices were dissected, separated from the hippocampus, and dissociated with a papain-based neural tissue dissociation kit (Milenyi Biotec) to obtain a single-cell suspension. ..

    Article Title: Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells
    Article Snippet: .. Single-cell suspensions were generated using a Tissue Dissociation Kit (Miltenyi Biotech, 130-097-043, Germany), with cell viability exceeding 90% as determined by trypan blue staining. ..

    Isolation:

    Article Title: IL-33 is Required for Behavioral Deficits in a Murine Model of Systemic Lupus Erythematosus Through Regulation of Microglia Glycolysis.
    Article Snippet: .. Single-cell suspensions were prepared from the isolated brain tissue using a papain-based neural tissue dissociation kit (Miltenyi Biotec), according to the manufacturer's instructions. .. Single-cell suspensions were prepared from the isolated brain tissue using a papain-based neural tissue dissociation kit (Miltenyi Biotec), according to the manufacturer's instructions.

    Suspension:

    Article Title: Astrocyte redox imbalance underlies prelimbic neuronal hypoactivity and maladaptive affective behaviors in epilepsy
    Article Snippet: .. Cortices were dissected, separated from the hippocampus, and dissociated with a papain-based neural tissue dissociation kit (Milenyi Biotec) to obtain a single-cell suspension. ..

    Generated:

    Article Title: Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells
    Article Snippet: .. Single-cell suspensions were generated using a Tissue Dissociation Kit (Miltenyi Biotech, 130-097-043, Germany), with cell viability exceeding 90% as determined by trypan blue staining. ..

    Staining:

    Article Title: Targeting Piezo1 can improve endometrial fibrosis by inhibiting ferroptosis in endothelial cells
    Article Snippet: .. Single-cell suspensions were generated using a Tissue Dissociation Kit (Miltenyi Biotech, 130-097-043, Germany), with cell viability exceeding 90% as determined by trypan blue staining. ..



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